LC3B抗体|Abcam中国|Anti-LC3B抗体(ab51520)
Your browser does not have JavaScript enabled and some parts of this website will not work without it.
为了使您在Abcam官网的浏览体验更顺畅，请使用最新版本的浏览器比如
我们使用cookies，让您更有效地浏览网站。请点击接受我们的cookies。如果您没有修改浏览器设置，我们假设您自动接受cookies。
Anti-LC3B抗体(ab51520)
Anti-LC3B抗体
兔多克隆抗体to LC3B
ab51520 is specific for the B isoform of LC3.
经测试应用
IHC - Wholemount, Flow Cyt, IHC-Fr, WB, ICC/IF
种属反应性
Mouse, Human
Synthetic peptide corresponding to Human LC3B (N terminal). Synthetic peptide made to the N terminal region of human LC3, isoform B protein.Database link:
WB: Treated U87-MG lysates.
ICC/IF: Treated U87-MG cells.
human malignant brain tumors, breast cancer, leukemia samples and murine cells
ab51520 has been shown to work on human malignant brain tumors, breast cancer, leukemia samples, and murine cells.
The product formulation changed to include 1% BSA on 17th May 2017. The following lot does not contain BSA and is still in stock GR320876. All future lots will contain 1% BSA.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA
Immunogen affinity purified
Primary antibody说明
ab51520 has been shown to work on human malignant brain tumors, breast cancer, leukemia samples, and murine cells.
Compatible Secondaries
Conjugation kits
Immunohistochemistry kits
Related Products
covers the use of
in the following tested applications.
The application notes include recommende optimal dilutions/concentrations should be determined by the end user.
IHC - Wholemount
Use at an assay dependent concentration.
1/3000. Predicted molecular weight: 15 kDa.
Detects bands of ~16 kDa (LC3-II) and ~18 kDa (LC3-I).
Probably involved in formation of autophagosomal vacuoles (autophagosomes).
组织特异性
Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.
序列相似性
Belongs to the MAP1 LC3 family.
翻译后修饰
The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.
Cytoplasm & cytoskeleton. Endomembrane system. Cytoplasmic vesicle & autophagosome membrane. LC3-II binds to the autophagic membranes.
数据库链接
ATG8F antibody
Autophagy-related protein LC3 B antibody
Autophagy-related ubiquitin-like modifier LC3 B antibody
Western blot - Anti-LC3B antibody (ab51520)
All lanes : Anti-LC3B antibody (ab51520) at 1/3000 dilutionLane 1 : treated U87-MG (human glioblastoma astrocytoma) lysates
with Arsenic Trioxide at 0 uMLane 2 : treated U87-MG (human glioblastoma astrocytoma) lysates
with Arsenic Trioxide at 2 uMLane 3 : treated U87-MG (human glioblastoma astrocytoma) lysates
with Arsenic Trioxide at 4 uMLane 4 : treated U87-MG (human glioblastoma astrocytoma) lysates
with Arsenic Trioxide at 8 uMPredicted band size : 15 kDa
Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody (ab51520)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling LC3B with&ab51520.An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry (Frozen sections) - Anti-LC3B antibody (ab51520)
Ab51520 at 1/2000 dilution staining treated U87-MG cultured and subcutaneous tumors
1) Control
2) Temozolomide
Flow Cytometry - Anti-LC3B antibody (ab51520)
Flow cytometry analysis of NTERA-2 cells with ab51520 at 1/200 using Dylight-488 conjugated goat anti-rabbit IgG secondary.
Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody (ab51520)
Ab51520 at 1/2000 dilution staining treated U373-MG 1) Control
2) C2-ceramide.
The nuclei were stained with DAPI.
Immunohistochemistry (Frozen sections) - Anti-LC3B antibody (ab51520)
Ab51520 at 1/2000 dilution staining treated U373-MG (human glioblastoma) cells
1) Control
2) Temozolomide
Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody (ab51520)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells using anti-LC3B antibody(red) at 0.1 ug/ml. Nuclei were counterstained with DAPI (blue).Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry (Frozen sections) - Anti-LC3B antibody (ab51520)
Ab51520 at 1/2000 dilution staining gliobastoma multiform tissue
1) normal brain
2) gliobastoma multiform tissue
Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody (ab51520)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells using anti-LC3B antibody(red). Nuclei were counterstained with DAPI (blue).
This product has been referenced in:
Wang X &et al. Role of hypoxia-inducible factor-1a in autophagic cell death in microglial cells induced by hypoxia. Mol Med Rep 15: (2017).
Cho JH &et al. Downregulation of SIRT1 signaling underlies hepatic autophagy impairment in glycogen storage disease type Ia. PLoS Genet 13:e17).
23 reviews or Q&A
Abcam community
Verified customer
Thank you for your reply.
I will be pleased to provide an free of charge vial of from a different lot.
Regrettably, this antibody is not in stock at the moment. Another batch is in production and is expected to be available by the...
Abcam Scientific Support
Application
Western blot
Monkey Tissue lysate - whole (Cardiac)
Gel Running Conditions
Reduced Denaturing
Loading amount
Specification
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) & Concentration: 5% & Temperature: 4&C
Abcam user community
Verified customer
this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Human Cell (Cardic stem celll)
Permeabilization
Yes - Triton x-100, 0.01%
Specification
Cardic stem celll
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) & Concentration: 1% & Temperature: RT&C
Formaldehyde
Abcam user community
Verified customer
Application
Immunocytochemistry
Mouse Cultured Cells (Cardiomyocytes)
Permeabilization
Yes - Triton x-100, 0.01%
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) & Concentration: 1% & Temperature: 4&C
Paraformaldehyde
Abcam user community
Verified customer
Application
Immunohistochemistry (Frozen sections)
Human Tissue sections (Heart)
Permeabilization
Yes - Triton x-100, 0.01%
Specification
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) & Concentration: 1% & Temperature: RT&C
Formaldehyde
Abcam user community
Verified customer
Application
Flow Cytometry
Mouse Cell (Cardiomyocytes)
Permeabilization
Yes - Triton X-100, 0.01%
Gating Strategy
LC3B-AlexaFluor488 positive
Specification
Cardiomyocytes
Preparation
Cell harvesting/tissue preparation method: TrypsinizationSample buffer: 0.25% for 30 sec
Paraformaldehyde
Abcam user community
Verified customer
Application
Western blot
Human Cell lysate - whole cell (Heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
Specification
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) & Concentration: 5%
Abcam user community
Verified customer
Application
Western blot
Mouse Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) & Concentration: 5% & Temperature: RT&C
Abcam user community
Verified customer
Application
Immunocytochemistry
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) & Concentration: 1%
Human Cultured Cells (HEK293)
Specification
Permeabilization
Yes - Triton x-100, 0.01%
Formaldehyde
Abcam user community
Verified customer
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) & Concentration: 3% & Temperature: 25&C
Mouse Cell (RAW cell)
Specification
Permeabilization
Yes - 3% BSA in PBS with 0.1% Triton X-100
Paraformaldehyde
Dr. Mukesh Jaiswal
Verified customer
1-10 of 23 Abreviews or Q&A
Please note: All products are &FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE&
通过邮件收到学术资源和促销信息基本参数/戴尔AximX51520MHZ
戴尔& 主要性能操作系统 Microsoft& Windows MobileTM 5.0操作系统 FOR Pocket PC，含Windows移动媒体播放器10&&处理器类型 处理器主频(MHz) 520MHz&&显示屏 TFT色彩16位，Transflective3.5英寸240 x 320分辨率，65,536色彩(QVGA)&&RAM容量（MB） 64MB SDRAM&&ROM容量（MB） ROM: 128MB闪存&&音频 音频控制器I2S Codec, WM8750声卡立体声转换16位立体声; 8.0, 11.025, 22.05和44.1KHz采样率和播放和扬声器&&
扩展插槽/戴尔AximX51520MHZ
&1个CompactFlashII类卡插槽(3.3v)1个数字安全/ SDIO Now / MMC存储卡插槽(3.3v)&&通讯端口/各类接口 标准v1.2 (115 kbps)红外端口36针支架/同步接口3.5毫米耳机/头戴设备插孔&&电池 1100mAh锂离子可拆卸、可充电电池(标配)&&电源 AC适配器&&外形尺寸 119*73*16.9&&产品重量 167g&&
随机附件/戴尔AximX51520MHZ
&可选: 1100mAh第二块电池可选: 2200mAh高容量电池可选: 真皮携带包可选: 带有无线技术的专用键盘&&
其他参数/戴尔AximX51520MHZ
&控制和灯光5向浏览按钮无线关闭/打开按钮锁开关4个程序键: 日历、联系人、收件箱、首页录音钮重置钮背光开关钮事件通知充电状态无线状态集成802.11b集成 无线技术&&
显示方式： |
共有12个词条
&|&相关影像
互动百科的词条（含所附图片）系由网友上传，如果涉嫌侵权，请与客服联系，我们将按照法律之相关规定及时进行处理。未经许可，禁止商业网站等复制、抓取本站内容；合理使用者，请注明来源于。
登录后使用互动百科的服务，将会得到个性化的提示和帮助，还有机会和专业认证智愿者沟通。
此词条还可添加&
编辑次数：5次
参与编辑人数：5位
最近更新时间： 18:50:11
贡献光荣榜
扫码下载APPAnti-LC3B antibody (ab51520) | Abcam
Your browser does not have JavaScript enabled and some parts of this website will not work without it.
For the best experience on the Abcam website please upgrade to a modern browser such as
We use cookies to make our site as useful as possible.
If you continue without changing your settings we'll assume you’re happy.
Your email
Message (optional)
Send me a copy of this email
I agree to the
Anti-LC3B antibody (ab51520)
Product name
Anti-LC3B antibody
Description
Rabbit polyclonal to LC3B
Specificity
ab51520 is specific for the B isoform of LC3.
Tested applications
Suitable for:
IHC - Wholemount, Flow Cyt, IHC-Fr, WB, ICC/IF
Species reactivity
Reacts with:
Mouse, Human
Synthetic peptide corresponding to Human LC3B (N terminal). Synthetic peptide made to the N terminal region of human LC3, isoform B protein.Database link:
Positive control
WB: Treated U87-MG lysates.
ICC/IF: Treated U87-MG cells.
human malignant brain tumors, breast cancer, leukemia samples and murine cells
General notes
ab51520 has been shown to work on human malignant brain tumors, breast cancer, leukemia samples, and murine cells.
The product formulation changed to include 1% BSA on 17th May 2017. The following lot does not contain BSA and is still in stock GR320876. All future lots will contain 1% BSA.
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium azideConstituents: PBS, 1% BSA
Immunogen affinity purified
Primary antibody notes
ab51520 has been shown to work on human malignant brain tumors, breast cancer, leukemia samples, and murine cells.
Polyclonal
Research areas
Compatible Secondaries
Conjugation kits
Immunohistochemistry kits
Related Products
covers the use of
in the following tested applications.
The application notes include recommende optimal dilutions/concentrations should be determined by the end user.
Application
IHC - Wholemount
Use at an assay dependent concentration.
1/3000. Predicted molecular weight: 15 kDa.
Detects bands of ~16 kDa (LC3-II) and ~18 kDa (LC3-I).
Probably involved in formation of autophagosomal vacuoles (autophagosomes).
Tissue specificity
Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.
Sequence similarities
Belongs to the MAP1 LC3 family.
Post-translationalmodifications
The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.
Cellular localization
Cytoplasm & cytoskeleton. Endomembrane system. Cytoplasmic vesicle & autophagosome membrane. LC3-II binds to the autophagic membranes.
Database links
Alternative names
ATG8F antibody
Autophagy-related protein LC3 B antibody
Autophagy-related ubiquitin-like modifier LC3 B antibody
Western blot - Anti-LC3B antibody (ab51520)
All lanes : Anti-LC3B antibody (ab51520) at 1/3000 dilutionLane 1 : treated U87-MG (human glioblastoma astrocytoma) lysates
with Arsenic Trioxide at 0 uMLane 2 : treated U87-MG (human glioblastoma astrocytoma) lysates
with Arsenic Trioxide at 2 uMLane 3 : treated U87-MG (human glioblastoma astrocytoma) lysates
with Arsenic Trioxide at 4 uMLane 4 : treated U87-MG (human glioblastoma astrocytoma) lysates
with Arsenic Trioxide at 8 uMPredicted band size : 15 kDa
Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody (ab51520)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling LC3B with&ab51520.An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemistry (Frozen sections) - Anti-LC3B antibody (ab51520)
Ab51520 at 1/2000 dilution staining treated U87-MG cultured and subcutaneous tumors
1) Control
2) Temozolomide
Flow Cytometry - Anti-LC3B antibody (ab51520)
Flow cytometry analysis of NTERA-2 cells with ab51520 at 1/200 using Dylight-488 conjugated goat anti-rabbit IgG secondary.
Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody (ab51520)
Ab51520 at 1/2000 dilution staining treated U373-MG 1) Control
2) C2-ceramide.
The nuclei were stained with DAPI.
Immunohistochemistry (Frozen sections) - Anti-LC3B antibody (ab51520)
Ab51520 at 1/2000 dilution staining treated U373-MG (human glioblastoma) cells
1) Control
2) Temozolomide
Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody (ab51520)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells using anti-LC3B antibody(red) at 0.1 ug/ml. Nuclei were counterstained with DAPI (blue).Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry (Frozen sections) - Anti-LC3B antibody (ab51520)
Ab51520 at 1/2000 dilution staining gliobastoma multiform tissue
1) normal brain
2) gliobastoma multiform tissue
Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody (ab51520)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells using anti-LC3B antibody(red). Nuclei were counterstained with DAPI (blue).
This product has been referenced in:
Wang X &et al. Role of hypoxia-inducible factor-1a in autophagic cell death in microglial cells induced by hypoxia. Mol Med Rep 15: (2017).
Cho JH &et al. Downregulation of SIRT1 signaling underlies hepatic autophagy impairment in glycogen storage disease type Ia. PLoS Genet 13:e17).
23 reviews or Q&A
Abcam community
Verified customer
Thank you for your reply.
I will be pleased to provide an free of charge vial of from a different lot.
Regrettably, this antibody is not in stock at the moment. Another batch is in production and is expected to be available by the...
Abcam Scientific Support
Application
Western blot
Monkey Tissue lysate - whole (Cardiac)
Gel Running Conditions
Reduced Denaturing
Loading amount
Specification
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) & Concentration: 5% & Temperature: 4&C
Abcam user community
Verified customer
this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Human Cell (Cardic stem celll)
Permeabilization
Yes - Triton x-100, 0.01%
Specification
Cardic stem celll
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) & Concentration: 1% & Temperature: RT&C
Formaldehyde
Abcam user community
Verified customer
Application
Immunocytochemistry
Mouse Cultured Cells (Cardiomyocytes)
Permeabilization
Yes - Triton x-100, 0.01%
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) & Concentration: 1% & Temperature: 4&C
Paraformaldehyde
Abcam user community
Verified customer
Application
Immunohistochemistry (Frozen sections)
Human Tissue sections (Heart)
Permeabilization
Yes - Triton x-100, 0.01%
Specification
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) & Concentration: 1% & Temperature: RT&C
Formaldehyde
Abcam user community
Verified customer
Application
Flow Cytometry
Mouse Cell (Cardiomyocytes)
Permeabilization
Yes - Triton X-100, 0.01%
Gating Strategy
LC3B-AlexaFluor488 positive
Specification
Cardiomyocytes
Preparation
Cell harvesting/tissue preparation method: TrypsinizationSample buffer: 0.25% for 30 sec
Paraformaldehyde
Abcam user community
Verified customer
Application
Western blot
Human Cell lysate - whole cell (Heart)
Gel Running Conditions
Reduced Denaturing
Loading amount
Specification
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) & Concentration: 5%
Abcam user community
Verified customer
Application
Western blot
Mouse Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) & Concentration: 5% & Temperature: RT&C
Abcam user community
Verified customer
Application
Immunocytochemistry
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) & Concentration: 1%
Human Cultured Cells (HEK293)
Specification
Permeabilization
Yes - Triton x-100, 0.01%
Formaldehyde
Abcam user community
Verified customer
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) & Concentration: 3% & Temperature: 25&C
Mouse Cell (RAW cell)
Specification
Permeabilization
Yes - 3% BSA in PBS with 0.1% Triton X-100
Paraformaldehyde
Dr. Mukesh Jaiswal
Verified customer
1-10 of 23 Abreviews or Q&A
PreviousNext
Please note: All products are &FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE&
Get resources and offers direct to your inbox