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低噪声高稳定度晶体振荡器的研究
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3秒自动关闭窗口术士2：放逐
Warlock 2: the Exiled
排名：第1296名
人气：加载中...
玩家评分：
最低配置推荐配置系统：Windows Vista x64/Windows 7 x64
CPU：2.66GHz双核CPU
内存：4GB以上内存
显卡：NVidia GeForce 8600 GT / ATI Radeon HD 6570
硬盘：2G以上空间
声卡：Direct Sound 兼容声卡系统：Windows Vista x64/7 x64/8 x64
CPU：Intel I3- GHz / AMD Phenom II X4 955 3.2 GHz
内存：4GB以上内存
显卡：NVidia GeForce 550 Ti / ATI Radeon HD 6850
硬盘：2G以上空间
声卡：Direct Sound 兼容声卡
《术士2：放逐》是一部回合制策略游戏，你将使用古老的法术和信仰作为武器，率领强大的军队投入激烈的战斗当中。
第三人称射击TPS(英文)7.3角色扮演RPG(英文)8.5即时战略RTS(中文)7.5角色扮演RPG(中文)7.7动作游戏(英文)9.7第一人称射击FPS(英文)8.8动作游戏ACT(英文/中文)7.5动作游戏ACT(英文)8.5角色扮演(日文)9.5动作游戏ACT(英文)7.0
·06-15·04-15·04-11·04-10·04-10·03-25·03-20·03-17·03-17·11-27
·03-31·03-31·03-31·03-31·03-31·03-31·03-31·03-31
术士2：放逐&精品攻略集《术士2：放逐》系统详解，图文攻略教学
术士2：放逐&视频
术士2：放逐&攻略
·07-11·05-19·05-08·05-08·05-06·05-04·05-04·04-30·04-29·04-25·04-25·04-25·04-23·04-22
术士2：放逐&下载
游戏下载&04-25&04-25&06-14&03-30
游戏补丁&07-26&10-23&06-19&06-15修改器&10-23&05-23&04-12&04-11游戏其他&07-26&06-19&06-15&06-12
术士2：放逐&截图
《术士2：放逐》高清截图（1）1/11《术士2：放逐》高清截图（2）2/11《术士2：放逐》高清截图（3）3/11《术士2：放逐》高清截图（4）4/11《术士2：放逐》高清截图（5）5/11《术士2：放逐》高清截图（6）6/11《术士2：放逐》高清截图（7）7/11《术士2：放逐》高清截图（8）8/11《术士2：放逐》高清截图（9）9/11《术士2：放逐》高清截图（10）10/11《术士2：放逐》高清截图（11）11/11
《术士2：娜迦之怒》游戏截图
《术士2：放逐》游戏中文截图
术士2：放逐&壁纸
热门网页游戏推荐
术士全系列
术士2：放逐&常见问题
&&&&&&&&&&
术士2：放逐&评论
[北京网友]
相比前作，1、很好的限制住了单位数量（因为限制了城的数量），不会让每一回合都变得手累眼累；2、自定义的魔法附加效果颇有意思；3、中立和野兽的存在感暴增，从“杂兵”升为“妨碍”；4、在小部分兵种的高伤害威胁下，让侦查变得更为重要；5、单位间的连携防御让守方更有利，于是攻方需要更多战术或更强有力的优势才行。总结：比前作减少了繁杂的操作，增加了自定义的乐趣，提高了战术战略的要求~
[四川省达州市电信网友]
直接不要MOD不行吗？？
[四川省达州市电信网友]
很好的游戏，虽然画面比不上《文明》，但个人感觉内容丰富，可玩性比文明高很多....
[泉州网友]
字体太小看的眼累
[苏州市网友]
12号补丁有汉化吗？
[27.10.4***]
进入游戏，显示不能加载mod？如何解决？
[北京网友]
相比前作，1、很好的限制住了单位数量（因为限制了城的数量），不会让每一回合都变得手累眼累；2、自定义的魔法附加效果颇有意思；3、中立和野兽的存在感暴增，从“杂兵”升为“妨碍”；4、在小部分兵种的高伤害威胁下，让侦查变得更为重要；5、单位间的连携防御让守方更有利，于是攻方需要更多战术或更强有力的优势才行。总结：比前作减少了繁杂的操作，增加了自定义的乐趣，提高了战术战略的要求~
[113.140***]
为什么没有评论呢7.2痛苦术士 橙披覆盖时的伤害增益分析
14:19:33 条 来源：TG兽吧 作者：清风善堂 ]
7.2痛苦术士 橙披覆盖时的伤害增益分析
　　基础知识
　　技能伤害系数
　　腐蚀术：14秒内254.1%sp，共7跳。每跳36.3%sp。
　　痛楚：18秒内214.4%sp伤害，共9跳。第一跳8%，之后每跳增加3.97%，之后每一条都是(8+3.97*8)=39.8%sp。
　　痛苦无常： 8秒内386.4%sp 共4跳。每跳96.6%sp。
　　吸取灵魂：6秒内720%sp，共6跳。每跳120%sp。
　　暴击：造成200%伤害
　　开启的基础伤害加成：10%
　　会影响伤害的神器特质
　　晶化暗影：2%
　　暗影白热：4%
　　已验证以上两条加法作用，即伤害加成系数=1+0.02+0.04=1.06
　　油尽灯枯：吸取灵魂伤害提高8%
　　丑恶腐蚀：腐蚀术提高16%
　　无比痛楚：痛楚提高16%
　　已验证以上三条和精通乘法作用，即伤害加成系数=(1+精通百分比)*(1+特质百分比)
　　无常永在：UA暴击提高8%
　　永劫不复：痛楚/腐蚀/UA/吸取灵魂的暴击伤害提高12%经区大师群卡老师指点，此处在点了三点的情况下应为20%，开神器后为40%，已经测试验证。
　　风选：持续伤害暴击提高4%
　　致命回响：UA结束后6%概率重新释放。
　　延绵恐惧：痛楚/腐蚀造成伤害时10%概率使下一个UA造成32%sp伤害。开神器后概率翻倍，伤害不翻倍(但被其他特质buff的部分翻倍)。
　　吞噬之怒：每杀死一个目标，提高2%dot伤害，可叠加5层，持续20秒。
　　这三条不纳入计算。
　　每个dot技能的期望伤害
　　基础伤害*(1+面板精通)*(1+旧巅峰+新巅峰)*(1+特质增加暗影伤害)*(1+特质增伤)*[(面板暴击+特质增加暴击)*(200%+特质增加暴击伤害)+(1-面板暴击-特质增加暴击)]*(1+传染)*(1+灾难之握天赋)
　　将所有特质和影响因素录入下表，加粗部分为会被神器覆盖翻倍的数值。
　　代入所有数值后，算得以下表格：
　　未开神器
　　开神器
　　人物模型/算前假设
　　1智力(sp)，0急速，20%暴击，0全能，120%精通。
　　为何选用1智力模型?
　　因为所有技能伤害都直接和sp挂钩，在计算开神器比不开神器增加的伤害百分比时，分子分母都含sp，所以约掉了。换句话说sp多少都不影响最终的增伤比例。
　　为何选用0急速模型?
　　因为所有dot持续时间/gcd时间/读条时间都是t/(1+面板急速)，和上述理由相同，因为开不开神器不影响急速，所以(1+面板急速)是一个可以约掉的固定值。当然了我现在用的计算模型是30秒总时长，单位时长0.5秒，如果一定要加入急速，那么总时长修改为(30/(1+面板急速))，单位时长修改为(0.5/(1+面板急速))即可，结果是一样的。
　　场景：30秒输出模拟。1-10层痛楚+腐蚀+2层UA读条+吸取灵魂
　　几个假设
　　30秒内痛楚一直是10层。
　　痛楚和腐蚀都是从一开始就存在于目标身上的。但是第0秒就开始补痛楚，紧接着补腐蚀，然后2UA吸魂。
　　30秒内痛楚不断，15秒的时候补了一次。腐蚀也不断(两次施法之间正好间隔14秒)。
　　致命回响，延绵恐惧，吞噬之怒并未计算在内。计算了这三个，开神器与不开神器的伤害差距会进一步拉大。
　　场景一
　　30秒平稳输出，输出手法为保持10层痛楚+腐蚀，两连读UA，无损吸魂。
　　红色蓝色：每一跳dot的间隔。
　　黑色：GCD
　　绿色：造成伤害的时间点。
　　橙色：由于延迟的存在，导致虽然&dot生效&和&吸取灵魂施放&同时发生，但是其实dot是吃不到灾难之握的。同理，当&dot生效&和&吸取灵魂结束&同时发生时，dot就可以吃到灾难之握，相当于dot整体吸取灵魂提前了等于延迟的毫秒数。这一点我已经在50ms的世界延迟下验证过了。
　　在以上模型中，数得dot伤害情况如下
　　结合之前的伤害表格，算得
　　未开神器总伤害为%sp
　　神器全程覆盖的总伤害为%sp
　　带来的伤害增加比例至少为37.39%。
　　场景二
　　30秒无UA输出。
　　之所以做这个场景二的模拟，是因为最近术士区有很多&神器不覆盖UA就没什么意义&的言论，此处手动滑稽。四件套术士正常输出循环是不会遇到这种极端情况的。
　　在以上模型中，数得dot伤害如下
　　结合之前的伤害表格，算得
　　未开神器总伤害为%sp
　　神器全程覆盖的总伤害为%sp
　　带来的伤害增加比例至少为38.19%
　　神器覆盖相比于不开神器
　　在30秒平稳输出模拟中(保持10层痛楚+腐蚀，2连读UA后无损吸魂)，带来的伤害增加比例至少为37.39%。
　　在30秒无UA输出模拟中(只保持10层痛楚+腐蚀，然后吸吸吸)，带来的伤害增加比例至少为38.19%。
　　那些纠结披风是不是不如项链/腰带/护腕的，希望这个帖子能够帮到你
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Peer-reviewed
Research Article
Novel Immunocytokine IL12-SS1 (Fv) Inhibits Mesothelioma Tumor Growth in Nude Mice
Novel Immunocytokine IL12-SS1 (Fv) Inhibits Mesothelioma Tumor Growth in Nude Mice
Heungnam Kim,&
Mitchell Ho
AbstractMesothelin is a glycosylphosphatidylinositol-anchored glycoprotein that is highly expressed on the cell surface of malignant mesothelioma. Monoclonal antibodies against mesothelin are being evaluated for the treatment of mesothelioma. Immunocytokines represent a novel class of armed antibodies. To provide an alternative approach to current mesothelin-targeted antibody therapies, we have developed a novel immunocytokine based on interleukin-12 (IL12) and the SS1 Fv specific for mesothelin. IL12 possesses potent anti-tumor activity in a wide variety of solid tumors. The newly-developed recombinant immunocytokine, IL12-SS1 (Fv), was produced in insect cells using a baculovirus-insect cell expression system. The SS1 single-chain Fv was fused to the C terminus of the p35 subunit of IL12 through a short linker (GSADGG). The single-chain IL12-SS1 (Fv) immunocytokine bound native mesothelin proteins on malignant mesothelioma (NCI-H226) and ovarian (OVCAR-3) cells as well as recombinant mesothelin on A431/H9 cells. The immunocytokine retained sufficient bioactivity of IL12 and significantly inhibited human malignant mesothelioma (NCI-H226) grown in the peritoneal cavity of nude mice and showed comparable anti-tumor activity to that of the SS1P immunotoxin. IL12-SS1 (Fv) is the first reported immunocytokine to mesothelin-positive tumors and may be an attractive addition to mesothelin-targeted cancer therapies.
IntroductionMesothelioma is an asbestos-related cancer that develops from transformed cells originating in the mesothelium. Mesothelin is a differentiation antigen of which expression in normal human tissues is limited to mesothelial cells lining the pleura, pericardium and peritoneum [,]. It is highly expressed in several human cancers including mesotheliomas, pancreatic cancers, ovarian cancers, lung adenocarcinomas, intrahepatic cholangiocarcinoma and breast cancer [-]. The mesothelin gene (MSLN) encodes a 71-kilodalton (kDa) precursor protein that is processed to a 40-kDa protein termed mesothelin, a glycosyl-phosphatidylinositol-anchored glycoprotein present on the cell surface [,]. Mesothelin has been suggested as a promising candidate for targeted therapy of multiple cancers [].
An immunotoxin, SS1P, that targets mesothelin-expressing tumors has been developed at the US National Cancer Institute (NCI) (Bethesda, MD) and is currently being evaluated in clinical trials []. Mice were immunized with a eukaryotic expression vector coding for mesothelin. When high serum antibody titers were obtained, a phage display library was made from the spleen mRNA of these mice. A single-chain variable fragment (scFv)-displaying phage (called SS) was selected that specifically bound to recombinant mesothelin and mesothelin-positive cells []. The SS Fv was further improved by in vitro affinity maturation [] and developed as the SS1P immunotoxin []. It contains a murine SS1 Fv fused to a 38-kDa fragment of Pseudomonas exotoxin A (PE38). Two Phase I clinical studies have been completed at the NCI [,].
Interleukin-12 (IL12) is a disulfide-linked heterodimeric glycoprotein consisting of a 35-(p35) and a 40-(p40)-kDa subunit []. IL12 can enhance the activation of natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), promoting the production of Interferon-gamma (IFN-γ), and inducing the differentiation of T helper cells [,]. IL12 also has potent antitumor, anti-angiogenic, and anti-metastatic activities. However, systemic administration of IL12 is thought to be highly toxic, particularly in multiple high doses []. In recent years, Dario Neri and colleagues developed IL12-based immunocytokines [-]. Delivery of IL12 by a tumor-specific antibody may achieve high and localized IL12 concentrations in the tumor microenvironment, and thereby stimulate and expand the immune effector cells sufficiently to the tumors without causing severe side effects. We think that this strategy could concentrate IL12 in the tumor microenvironment and thereby enhance the antitumor response, presenting an alternative approach for treating patients with mesothelin-overexpressing solid tumors. In this study, we found that IL12-SS1 (Fv) specifically bound mesothelin in several cancer cell lines.
We also determined that treatment of mesothelioma tumor-bearing nude mice with IL12-SS1 (Fv) drastically reduced tumor burden in these mice.
These results indicate that IL12-SS1 (Fv) may be an effective therapeutic for treating malignant mesothelioma in patients.
Materials and Methods
Ethics statement
All mice were housed and treated under the protocol (LMB-059) approved by the Institutional Animal Care and Use Committee at the National Institutes of Health (NIH).
Cell lines
The following cell-lines were used in this study: The human mesothelioma cell line NCI-H226, ovarian cancer cell OVCAR-3, and epidermoid carcinoma A431 were obtained from the American Type Culture Collection (ATCC, Rockville, MD). The embryonic kidney cell line, HEK-293, was purchased from Life Technologies (Grand Island, NY). A431/H9: the transfected A431 cell line stably expressing mesothelin was described previously []. The cell lines were maintained as adherent monolayer cultures in RPMI 1640 medium (Life Technologies, Gaithersburg, MD) supplemented with 10% fetal bovine serum (FBS) (HyClone, Logan, UT), 1% L-glutamine, and 1% penicillin/streptomycin (Life Technologies, Gaithersburg, MD) and incubated in 5% CO2 with a balance of air at 37 °C. Media was changed twice a week to examine the binding properties of IL12-SS1 (Fv). Cells were confirmed to be negative for mycoplasma. The previously generated human mesothelioma cell line LMB-H226-GL was used for the mouse xenograft model []. Briefly, we fluorescently labeled the NCI-H226 human mesothelioma cell line by a lentiviral vector harboring a luciferase-GFP (Luc/GFP) fusion gene driven by the RNA polymerase II promoter. After single-cell cloning by flow cytometry, a clone (named LMB-H226-GL) that stably expresses high levels of Luc/GFP was obtained.
Plasmid construction and cloning of IL12-SS1 (Fv)
Baculovirus cloning, expression, and purification of IL12-SS1 (Fv) was performed by Protein Expression Laboratory, ATP, SAIC-Frederick. pDonr253 is a Gateway Donor vector modified from pDonr201 (Life Technologies, Gaithersburg, MD). pDonr253 replaces the kanamycin resistance gene with a gene encoding spectinomycin resistance. The oligonucleotides used in this study are listed in .
PrimersNoteSequences9228p40 Forward5’-ATGTGGGAGCTGGAGAAAGACGTTTATG-3’9229p35 Forward5’-CCGATCCGGTGGCGGTGGCTCGGGCGGTGGTGGGTC GGGTGGCGGCGGATCTAGGGTCATTCCAGTCTCTGGACCTGCC-3’9230p40 Reverse5’-CCGACCCACCACCGCCCGAGCCACCGCCACCGGATC GGACCCTGCAGGGAACACATGCCC-3’9231SS1 Forward5’ – GGCTATCTGAGTTCCGCCGGAAGCGCTGATGGAGGT ATGGCCCAGGTGCAGCTGCAGCAG-3’9233SS1 Reverse5’-GCCCTTGTCGTCATCGTCCTTATAATCGCCCCGTTTTA TTTCCAACTTTGTCCCAGC-3’9234p40 Forward5’-CCATCTCCTGGTTTGCCATCGTTTTGCTGGTGTCTCCA CTCATGGCCATGTGGGAGCTGGAGAAAGACG-3’9235p40 Reverse5’-GGGGACAACTTTGTACAAGAAAGTTGATTAATGGTG ATGGTGATGGTGATGGTGGCCCTTGTCGTCATCGTCC-3’9237Adaptor5’-GGGGACAACTTTGTACAAAAAAGTTGGCACCATGTG TCCTCAGAAGCTAACCATCTCCTGGTTTGCCATCG-3’9330p35 Reverse5’-CGCTTCCGGCGGAACTCAGATAGCCCATCACCCTGTT GATGGTCACGACGCGGGTGCTGAAGGCG-3’Table 1.
Primers used for cloning of IL12-SS1 (Fv).
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The subunits of IL12 with SS1 were synthesized (Genescript, Piscataway, NJ) and cloned into pFuse vector (Invivogen, San Diego, CA). We made plasmids pFuse-p40-SS1 and pFuse-SS1-p35 containing p40 and p35 of IL12 with SS1, respectively. The IL12-SS1 (Fv) fragment was constructed using triple overlap PCR from pFuse-p40-SS1 and pFuse-SS1-p35. Three separate PCR reactions were carried out to amplify the IL12 p40, IL12 p35, and SS1 Fv fragments containing 24-28 bp overlapping sequences. Initial PCRs were carried out using Phusion DNA polymerase (New England Biolabs, Ipswich, MA) as noted in
and per manufacturer’s instructions. The PCR products were purified using the QiaQuick PCR purification kit (Qiagen, Valencia, CA), and equal amounts of the three products were mixed together in a final PCR reaction with the primers noted “IL12-SS1 (Fv)” in .
The p40 and p35 subunits of murine IL12 were connected with flexible linker (Ser4Gly)3. scFv (SS1) was fused to the p35 subunit of IL12 through flexible linker (GSADGG) and IL12-SS1 (Fv), which contains Flag and 8 His tag at the C-terminal. After five cycles of amplification, 200 nM of 9237 adapter primer was added and amplification was continued for 20 additional cycles. The adapter primer, which contains the attB1 site and part of the signal sequence, was used to attach the attB1 site during an adapter PCR process.&Conditions were the same as for the original PCR but with an extension time of 1 minute. The final PCR products were flanked by Gateway recombination signal sequences, attB1 at the 5’ end and attB2 at the 3’ end.
The PCR products were purified using the QiaQuick PCR purification kit (Qiagen, Germantown, MD), and recombined into pDonr253 with the Gateway BP recombination reaction kit following manufacturer’s instruction (Life Technologies, Gaithersburg, MD). E. coli DH10B cells were transformed with the BP reactions and colonies were isolated on LB plates containing 50 ug/ml of spectinomycin. The sequence-verified entry clone was subcloned by Gateway LR recombination (Life Technologies, Gaithersburg, MD) into pDest-8 for insect cell expression.
Final expression clones were verified by size and restriction digest pattern. E. coli DH10Bac (Life Technologies, Gaithersburg, MD) was then transformed with the expression clones and plated on selective media containing gentamycin, kanamycin, tetracycline, IPTG, and X-gal as per the manufacturer’s protocol. White colonies were selected from these plates, and bacmid DNA was recovered by alkaline lysis plasmid preparation and verified by PCR amplification across the bacmid junctions.
The expression of IL12-SS1 (Fv) in insect cells using baculovirus
The IL12-SS1 (Fv) protein was expressed in baculovirus-infected insect cells. Sf-9 cells were maintained in suspension cultures of Hyclone SFX-Insect medium (Thermo Scientific, Rockford, IL). One day prior to the large-scale direct transfection the Sf-9, cells were fed to ensure that the cells were in logarithmic growth.& On the day of transfection,100 ml of Sf-9 cells per transfection were set at 1.5 x106 cells/ml in a 490 cm2 roller bottle (Corning, Tewksbury, MA) and incubated (27°C at 100 rpm) in an INNOVA 4430 shaker (New Brunswick, NJ) while the transfection mixtures were prepared (30 - 60 minutes). Fifty microliters of each bacmid DNA was added to 200 ul of saline in one microcentrifuge tube and 150 ul of&transfection reagent (XpressNOW, Lonza, Germany) was added to 200 ul of saline in another tube.& The diluted bacmid DNAs were added to the diluted transfection reagent and the combinations were mixed gently by pipetting. The mixture was incubated for 10 minutes at room temperature and then added to the 100 ml Sf-9 suspension cultures and the bottles were immediately returned to the 27°C shaking incubator. After four days the cultures were centrifuged at 2000 rpm.& The supernatants containing the recombinant baculoviruses were stored at 4°C in the dark. For protein expression,&High Five (H5) cells (Life Technologies, Gaithersburg, MD) were fed the day before infection to insure the cells were in logarithmic growth phase the following day. One liter of H5 cells (1.5 x 106 cells/ml) in SFX medium was infected with 40 ml of the p40-p35-SS1 (Fv) virus. The culture was incubated at 21°C for 72 hr at an orbit speed of 100 rpm after which the supernatant was collected for purification.
Recombinant immunocytokine protein purification
One liter of clarified culture supernatant from baculovirus-infected H5 insect cells was concentrated to 200 ml using a 30 kDa normal molecular weight cutoff (MWCO) membrane (EMD Milipore, Billerica, MA), buffer exchanged to PBS, pH 7.2 (using tangential flow filtration), amended with 50 mM imidazole, and applied to a 5 ml Histrap HP column, (GE Healthcare) that had been equilibrated in PBS, pH 7.2, 50 mM imidazole, at a flow rate of 2 ml/min. The column was washed to baseline with equilibration buffer and proteins eluted in a 20-column volume gradient from 50-500 mM imidazole in PBS, pH 7.2 positive fractions (as identified by SDS-PAGE/Coomassie staining) were pooled and dialyzed to PBS, pH 7.2. Protein concentration was determined by the Bradford assay and bovine IgG as standard (Bio-Rad, Hercules, CA).
Flow cytometry
To determine binding of IL12-SS1 (Fv) to mesothelin on the cell surface, cancer cells (H226, OVACR-3, H9, A431, and 293-F cells) were grown until confluent, then harvested, washed, and resuspended in ice-cold PBS containing 5 % bovine serum albumin (BSA). Cells were incubated with 5 μg/mL of MN (mesothelin mAb; Rockland Immunochemicals, Gilvertsville, PA) or an isotype antibody and PE-conjugated Anti-Histidin (Abcam, Cambridge, MA). Binding was detected using goat anti-mouse IgG conjugated with phycoerythrin (Sigma-Aldrich, St. Louis, MO). The fluorescence associated with the cells was measured using a FACSCalibur flow cytometer (BD Biosciences, San Jose, CA).
To evaluate inhibition of the binding of IL12-SS1 (Fv) to A431 cells by an anti-IL12 receptor antibody, 0.5 x 106 A431 cells were incubated with 25 ug/mL of anti-IL12R β2 antibody (Thermo Scientific, Rockford, IL) and incubated on ice for 1 hour. Five ug/milliliter of SS1-IL12 was then added and incubated on ice for an additional hour. After washing, the cells were stained with goat anti-Flag-FITC conjugate (Abcam, Cambridge, MA) at a 1:200 dilution on ice for 1 hour. After washing, the fluorescence associated with the cells was measured by flow cytometry.
Nunc MaxiSorp 96-well flat-bottomed plates were incubated overnight with 10 to 5000 ng/ml of purified rabbit Fc MSLN (rFc-MSLN) or rabbit Fc control in PBS, followed by an hour block with 5% BSA, 0.01% NaN3 in PBS. Purified recombinant IL12-SS1 (Fv) was diluted to 1 μg/ml in ELISA buffer (0.01% Tween 20, 10% SuperBlock, Thermo Scientific, Rockford, IL) and incubated on a plate for 1 hour at room temperature. Plates were then incubated with goat anti-rabbit IgG conjugated HRP for 1 hour at room temperature. The plates were washed four times with ELISA buffer between each coating. Visualization was achieved with 3,3′,5,5′-tetramethylbenzidine detection reagent (KPL, Gaithersburg, MD), and the absorbance was read at 450 nm with a SpectraMax Plus plate reader (Molecular Devices, Sunnyvale, CA). Any absorbance values equal or less than 0.1 are considered negative.
The bioactivity of IL12-SS1 (Fv)
Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coat of health donors (Department of Transfusion Medicine, NIH Clinical Center) and activated with 10 ug/ml of PH-A for 4 days. Cell proliferation by IL12-SS1 (Fv) or IL12 was measured by a WST-8 colorimetric assay using the Cell Counting Kit-8 (Dojindo Molecular Technologies, Rockville, MD) according to the manufacturer's instructions. Two hundred microliters of PBMCs were seeded at 1 x 104 cells per well in a 96-well plate, and recombinant IL12 or the IL12-SS1 (Fv) fusion proteins were added at the indicated concentrations. Cells were incubated at 37°C for 72 hours, followed by a proliferation assay using Cell Counting Kit-8. The IL12 activity assay was performed with PBMCs in the presence of murine recombinant IL12 or IL12-SS1 (Fv) fusion proteins for 4 days by assessing its ability to induce IFN-γ production using a Human IFN-γ Quantikine ELISA Kit (R&D Systems, Minneapolis, MN).
Peritoneal mesothelioma mouse xenograft model
Eight-week old female athymic nude mice (ATHYMIC NCr-nu/nu) obtained from the Animal Production Program at the Frederick National Laboratory for Cancer Research (formerly NCI-Frederick) were housed in micro-isolator cages. To investigate the therapeutic effect of IL12-SS1 (Fv) against established mesothelioma, mesothelioma tumors were established by intraperitoneal (i.p.) injection of 5 million LMB-H226-GL cells in 200 ul of growth media into the low abdomen or flank area of nude mice following our previously reported lab protocol []. Animals were imaged the following day and then once every week thereafter. The animal were divided into 4 Vehicle, SS1P (0.4 mg/kg), IL12-SS1 (Fv) (0.4 mg/kg), or IL12-SS1 (Fv) (1.6 mg/kg body weight)-treated mice.
Animal treatment
We treated the tumor-bearing mice with IL12-SS1 (Fv), SS1P, or vehicle every the other day. The day when the mice were injected with the tumor cells was set as day 1. We used SS1P, an anti-MSLN scFv conjugated with pseudomonas toxin, as a positive control. The treatment group and control group each contained 3 mice for initial experiments and 5 mice for repeated experiments. Each mouse in the treatment groups received 0.4 mg/kg body weight of SS1P, 0.4 mg/kg (low dose group), or 1.6 mg/kg (high dose group) of IL12-SS1 (Fv) every other day. The control group received PBS as a vehicle control. Body weight and tumor growth were assessed twice a week.
Assessment of tumor growth
Two hundred microliters of 15 mg/mL D-luciferin (Caliper Life Sciences, Hanover, MD) in PBS was injected i.p. before imaging. The luciferase activity of the tumor was calculated using Living Image 3.1.0 software (Caliper Life Sciences, Hanover, MD). Intraperitoneal Tumor growth was assessed using photon intensity, photons per second (ph/sec) as luciferase activity following our lab protocol [].
In vivo toxicology studies
At the end of the treatment, three mice in each group were transferred to SAIC-Frederick (Frederick, MD) and euthanized. Blood was taken for whole blood complete blood counts (CBC) and serum chemistry analysis. A full necropsy was performed, in which organs and tissues were weighed and examined for gross findings.
Statistical analysis
Statistical analysis was performed with Prism (version 5) for Windows (GraphPad Software, La Jolla, CA). Raw data were analyzed by “analysis of variance” with Dunnett's and Newman-Keuls multiple comparison post tests. p values & 0.05 were considered statistically significant.
Results and DiscussionCytokines are key mediators of innate and adaptive immunity. Even though many cytokines have been used for cancer therapy in patients with advanced cancer, they can sometimes cause severe toxicity [-]. The use of ‘immunocytokines’, antibody-cytokine fusion proteins, has been proposed to overcome these problems. In this study, we aim to generate an immunocytokine based on the SS1 anti-mesothelin Fv and investigate whether it is a feasible option for treating mesothelioma and other mesothelin-expressing tumors.
Construction of the IL12-SS1 (Fv) immunocytokine
To generate a novel immmunocytokine targeting mesothelin, we constructed monomeric IL12-SS1 (Fv), which is a fusion of IL12 to the anti-mesothelin antibody fragment scFv (SS1). Gafner et al. reported comparative analysis of three fusion proteins (monomeric, heterodimeric and homodimeric protein) comprising scFv (L19) and IL12. Heterodimer protein, which has two subunits consisting of p40 and p35 fused to scFv, showed the most effective tumor uptake and displayed anti-tumor activity superior to monomeric IL12-scFv (L19) in xenograft mouse models []. In the present study, we decided to use a similar approach to construct IL12-SS1 (Fv). The p40 and p35 subunits of murine IL12 were connected by flexible linker (Ser4Gly)3. The scFv (SS1) was fused to the p35 subunit of IL12 through 6 amino acid flexible linker (GSADGG) and IL12-SS1 (Fv) which contains Flag and 8XHis tag at the C-terminal for detection. The final PCR products were flanked by Gateway recombination signal sequences, attB1 at the 5’ end and attB2 at the 3’ end ().
depicts the construct containing the p40 and p35 subunits of murine IL12 and the SS1 scFv. IL12-SS1 (Fv) was expressed in sf9 insect cells and fusion protein was purified to homogeneity by affinity chromatography. The production yields were 1.2 mg for IL12-SS1 (Fv) per liter of cell culture supernatant. The resulting purified IL12-SS1 (Fv) protein was characterized by SDS- PAGE analysis, confirming the presence of a single band of apparent molecular weight equal to 90 kDa under reducing and non-reducing conditions ().
Construction of IL12-SS1 (Fv) fusion protein.(A) The map of the baculovirus expression vector. IL12-SS1 (Fv) was inserted at the recombination sites attB1 and attB2 using the gateway cloning system. (B) Cloning strategy and schematic representation of the IL12-SS1 (Fv) fusion protein. The IL12-SS1 (Fv) fusion protein consists of p40 and p35 fused to scFv (SS1). (C) SDS-PAGE analysis of 5 ug of IL12-SS1 (Fv) protein under reducing (R) or non-reducing (NR) conditions confirms protein size. Analysis: SDS-PAGE, 4-20% gradient gel, Coomassie blue staining. M; protein standards, kDa.
IL12-SS1 (Fv) specifically binds mesothelin and exerts bioactivity similar to IL12
To evaluate the binding properties and functionality of the IL12-SS1 (Fv) immunocytokine, we conducted in vitro analysis using recombinant proteins and human cancer cells. The binding specificity of IL12-SS1 (Fv) for MSLN was examined by ELISA (). The recombinant IL12-SS1 (Fv) bound to MSLN in a dose-dependent manner. We used a rabbit Fc (rFc) fusion protein as a negative control and found there was no binding to rFc (OD &=0.1) and no dose-dependent correlation with rFc binding (). To determine whether the binding of IL12-SS1 (Fv) to the cells was specific, the in vitro binding capability of IL12-SS1 (Fv) to MSLN-expressing cells was assessed by flow cytometry (). Using IL12-SS1 (Fv) for detection, MSLN expression was determined to be strongly positive in NCI-H226, OVCAR-3, and H9 cells, and weakly positive in mesothelin-negative A431 and HEK-293 cells. In the presence of an IL12 receptor blocking antibody, the binding of IL12-SS1 (Fv) to A431 cells was inhibited, indicating that IL12-SS1 (Fv) binds to the IL12 receptor on A431 cells (). In particular, the binding was stronger in H9, an A431 line highly expressing MSLN on the cell surface, than in A431 which is mesothelin-negative. To determine the binding affinity of IL12-SS1 (Fv) for cell surface-associated MSLN, flow cytometric analysis was performed (). We used SS1P as a positive control and found that IL12-SS1 (Fv) and SS1P bound to H9 in a dose-dependent manner. The binding affinity (equilibrium Kd) of IL12-SS1 (Fv) and SS1P for mesothelin-expressing H9 cells were approximately 60 nM and 2.8 nM, respectively. The 20-fold loss of IL12-SS1 (Fv) binding affinity for mesothelin may attribute to the potential interference of single-chain IL12 with SS1. The SS1 single-chain Fv was fused to the C terminus of IL12 through a short linker (GSADGG). It may be worthwhile optimizing the linker sequence in future studies to retain the binding affinity of SS1 (Fv). It is also possible that folding and post-translational modification are different for the two molecules because we used insect cells to express immunocytokine IL12-SS1 (Fv) and E. coli for immunotoxin SS1P.
Binding of IL12-SS1 (Fv) to MSLN and cancer cells.(A) The binding specificity of IL12-SS1 (Fv) on MSLN was detected by ELISA. (B) FACS analysis of IL12-SS1 (Fv) on mesothelin-expressing cancer cells. NCI-H226, OVCAR-3, H9, A431, and HEK-293 cells were stained with IL12-SS1 (Fv) which was detected by PE-conjugated anti-histidine antibody (solid line). Mesothelin expression was detected by a mouse anti-MSLN antibody (MN, dotted line) or an irrelevant isotype mAb control (gray surface). Samples were analyzed by flow cytometry. H9: the transfected A431 cell line stably e OVCAR-3: human ovari NCI-H226: human mes HEK-293: human embryonic Kidney cell lines. (C) FACS analysis of IL12-SS1 (Fv) on A431 cells. A431 cells were stained with IL12-SS1 (Fv) in the presence of (dotted line) or the absence of (solid line) an anti-IL12R β2 blocking antibody. The binding of IL12-SS1 (Fv) was detected by FITC-conjugated anti-Flag antibody. (D) SS1P and IL12-SS1 (Fv) bound to H9 cells in a dose-dependent manner. H9 cells were stained with indicated concentrations of IL12-SS1 (Fv) and then detected by flow cytometry. The Kd value for SS1P and IL12-SS1 (Fv) binding to H9 cells are approximately 2.8 and 60 nM, respectively.
IL12 is a key immunoregulatory cytokine and plays an essential role in the interactions between the innate and adaptive arms of immunity by acting on natural killer (NK) cells and T cells and enhancing the generation and activity of cytotoxic lymphocytes [-]. IL12 is responsible for the priming of Th1 cell responses and the secretion of large amounts of IFN-γ from T cells and NK cells []. IL12 induces an antitumor response in a murine model of malignant mesothelioma []. IL12 has exhibited potent antitumor and anti-metastatic activity in preclinical studies [,-]. Clinical trials in patients with cancer have revealed promising therapeutic activities, but have also shown that recombinant human IL12 is extremely toxic to humans []. Since human IL12 has no biological activity in mice [], we used mouse IL12 in this study.
Several studies with tumor specific antibodies fused with IL12 have been reported, including: anti-CD30 antibody for Hodgkin’s lymphoma, anti-HER2 antibody for HER2-expressing tumors [,], and anti-extra-domain B (ED-B) of fibronectin for tumor vessels, IL12-huBC1 [], and IL12-L19 []. In order to confirm whether purified IL12-SS1 (Fv) maintains the biological activity of IL12, we compared the stimulatory effect of murine IL12 and IL12-SS1 (Fv) in a lymphocyte proliferation assay, as well as the ability of these proteins to induce INF-γ production in PBMC. Stimulation of PBMCs with doses of IL12 or IL12-SS1 (Fv) as low as 1 ng/ml caused an increase in proliferation (). The specific binding affinity of IL12-SS1 (Fv) to MSLN was 60 nM. Sommavilla et al. have reported about an immunocytokine consisting of the scFv (F8) specific to the extra-domain A of fibronectin and human IL12, F8-IL12,
with lymphoproliferative activity at 600 nM []. A significant dose-dependent increase in the induction of IFN-γ by treatment with either IL12 or IL12-SS1 (Fv) was noted (). IL12-SS1 (Fv) exhibited comparable biological activities, which was slightly lower than recombinant murine IL12 used as a standard, at high concentrations (100 ng/mL and 1000 ng/mL). At low concentrations (10 ng/mL or less), IL12-SS1 (Fv) exhibited 2- to 4-fold less activity as compared to the control IL12. Taken together, these data indicate that the recombinant IL12-SS1 (Fv) fusion protein exerts its bioactivity by proliferation and induction of IFN-γ production in PBMC.
In vitro functional analysis.Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coat and activated by 10 ug/ml of PH-A for 4 days. The IL12 activity assay was performed with PBMCs in the presence of murine recombinant IL12 (mIL12) or IL12-SS1 (Fv) fusion proteins for 4 days. (A) Cell proliferation was monitored by a colorimetric assay (WST-8). (B) INF-γ in the supernatant was detected by Human IFN-γ Quantikine ELISA Kit (R&D Systems).
IL12-SS1 (Fv) inhibits mesothelioma tumor growth in nude mice
To investigate the therapeutic effect of IL12-SS1 (Fv) against established mesothelioma, we performed a pilot study in mice. We inoculated mice with LMB-H226-GL (NCI-H226-GFP/Luciferase) mesothelioma cells (). In an initial animal experiment, we treated mice with two different doses of IL12-SS1 (Fv), 0.4 mg/kg and 1.6 mg/kg. After 13 days of tumor development, we treated the tumor-bearing mice at days 13, 15, 17, and 19 with 10 or 1.6 mg/kg of IL12-SS1 (Fv), 0.4 mg/kg body weight of SS1P, or a vehicle control. Tumor sizes were accessed via in vivo bioluminescence measurement using the IVIS Imaging System.
shows that the IL12-SS1 (Fv) and SS1P treatment groups exhibited significantly retarded tumor growth compared with the saline treatment group.
In mice treated with vehicle, the proton ranges were gradually increased to 0.8, 5.7 and 8.3 X 109 ph/sec at 18, 24, and 31 days, respectively after i.p. LMB-H226-GL injection. When photons were measured after injections with IL12-SS1 (Fv) or SS1P, the tumor size was significantly reduced. The photons were 0.4, 7.4, and 1.8 X 108 at 31 days after treatment of SS1P (0.4 mg/kg), IL12-SS1 (Fv) (0.4 mg/kg and 1.6 mg/kg), respectively. At Day 18, the tumor sizes in either SS1P or IL12-SS1 (Fv) treated groups were not significantly reduced as compared to the control group (p & 0.05). At Day 24 and Day 31, the tumor sizes in the mice treated with either SS1P or IL12-SS1 (Fv) were significantly reduced (p & 0.05). Interestingly, the anti-tumor activity of IL12-SS1 (Fv) was similar to that of SS1P (p & 0.05). High dose (1.6 mg/kg) or low dose (0.4 mg/kg) of IL12-SS1 (Fv) had similar tumor growth inhibition (p & 0.05), indicating that a low dose of the IL12-SS1 (Fv) immunotoxin may also be effective in vivo. No significant weight loss was observed in mice treated with vehicle, SS1P, or IL12-SS1 (Fv). In the next animal experiment, we used five mice in each group and 0.4 mg/kg of IL12-SS1 (Fv) (). As shown in the first experiment, both IL12-SS1 (Fv) and SS1P caused tumor remission at Day 21 while the control mice grew large tumors. Collectively, these results suggest that the therapeutic effect of IL12-SS1 (Fv) and its efficacy is comparable to that of the SS1P immunotoxin.
Tumor therapy studies with IL12-SS1 (Fv).(A) Treatment of 8-week old female athymic nude mice with IL12-SS1 (Fv) after intraperitoneal injection of LMB-H226-GL. SS1P (0.4 mg/kg), IL12-SS1 (Fv) (0.4 mg/kg), IL12-SS1 (Fv) (1.6 mg/kg), or vehicle (PBS) were intraperitoneally injected times at days 13, 15, 17, and 19 post-injection. Tumor growth was measured by bioluminescence photometry at day 18, 21, and 31. The photometry of the in vivo imaging was acquired using Living Image 3.1.0 software (Caliper Life Sciences). *Indicates the significant (p & 0.05) difference between treatment and control (vehicle) by using the one-way ANOVA statistical test (GraphPad Prism 5.03). (B) Photograph of representative mice treated with IL12-SS1 (Fv), SS1P and vehicle (PBS).
To examine the toxicity of IL12-SS1 (Fv), we performed in vivo toxicology studies at the end of the treatment (). Blood from mice in treatment and control groups (three mice per group) was analyzed by CBC, and sera isolated for serum chemistry analyses. All serum chemistry and CBC in the IL12-SS1 (Fv)-treated group were similar to those of the control group exce the organ weights of IL12-SS1 (Fv) treated-mice were not significantly different from those of the control group mice, indicating that IL12-SS1 (Fv) has no serious toxicity in mice. We noted that the white blood cells were elevated by about 2-old in the IL12-SS1 (Fv) group (16.28 ± 2.47 K/uL) as compared to the control group (7.1 ± 2.16 K/uL) and the SS1P group (7.51 ± 0.97 K/uL). A larger scale study will be needed to investigate the pharmacokinetics, pharmacodynamics and bio-distribution of IL12-SS1 (Fv).
Complete blood counts and serum chemistry ResultsSelected parametersControlSS1PIL12-SS1 (Fv)&&&Normal valuesWhite blood cells (K/uL)7.1 ± 2.167.51 ± 0.9716.28 ± 2.47 1.80 - 10.70Red blood cells (M/uL)9.47 ± 0.329.51 ± 0.0210.35 ± 0.84 6.36 - 9.42Albumin (g/dL)4.4 ± 0.424.27 ± 0.424.1 ±
0.3 1.6 - 2.8Alkaline phosphatase (U/L)60.5 ± 9.19 13.0154 ± 15.1373 ±
4.36 67 - 282Alanie aminotransferase (U/L)61 ± 16.52 16.1746.33 ± 4.5146.67 ±
12.50 29 - 181Total bilirubin (mg/dL)0.30.30.37 ±
0.06 0.0 - 0.6Creatinine (mg/dL)&0.2&0.2&0.20.2 - 0.4Hemoglobin (g/dL)14.53 ± 0.4214.47 ± 0.6514.83 ± 0.64 11.00 - 15.10Total protein (g/dL)6.67 ± 0.646.47 ± 0.156.13 ±
0.15 4.2 - 5.9Blood urea nitrogen (mg/dL)25.67 ± 3.0625 ± 2.6523 ±
1.7312 - 52Organ weights (mg)Brain0.45 ± 0.040.43 ± 0.020.43 ± 0.02NAHeart0.12 ± 0.020.12 ± 0.010.14 ± 0.01NAKidney0.31 ± 0.040.29 ± 0.020.33 ± 0.02NALiver1.02 ± 0.291.01 ± 0.051.17 ± 0.09NALung0.16 ± 0.030.16 ± 0.040.16 ± 0.01NASpleen0.14 ± 0.120.12 ± 0.020.12 ±0.06NATable 2.
Selected in vivo toxicological results and organ weights.
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In conclusion, we described an IL12-based immunocytokine that targets cell surface-associated mesothelin proteins in mesothelioma. The recombinant immunocytokine produced in baculovirus-insect cell expression system is as biologically active as IL12 alone. Furthermore, our animal testing showed that the immunocytokine exhibited inhibition of human malignant mesothelioma grown in the peritoneal cavity of nude mice. Our results may lead to a new mesothelin-targeted antibody conjugate, which is different from other anti-mesothelin antibodies (such as chimeric antibody MORAb-009) or immunotoxins (SS1P) currently being evaluated in clinical studies. IL12-SS1 (Fv) presents a significant advantage given that local delivery of IL12 to tumor microenvironment by the anti-mesothelin antibody may enhance specific immune response against tumors. Recently, several anti-mesothelin human monoclonal antibodies have been developed [-]. Therefore, it is possible to make fully human IL12-based immunocytokines to mesothelin for the treatment of mesothelioma and other mesothelin-expressing tumors in patients.
Acknowledgments
The authors thank Ira Pastan (NCI) for helpful discussions and providing the SS1P immunotoxin, Mingqian Feng (NCI) for technical assistance in LMB-H226-GL animal testing, Protein Expression Laboratory, SAIC-Frederick, Inc. for producing IL12-SS1 (Fv) in baculovirus-insect cell expression system, particularly Dominic Esposito for cloning, Ralph (Butch) Hopkins for baculovirus expression, and William Gillette for protein purification, Miriam Anver and Donna Butcher (Pathology/Histotechnology Laboratory, SAIC-Frederick, Inc.) for in vivo toxicity test and pathological analysis. We also thank the NIH Fellows Editorial Board and Yen Phung (NCI) for editorial assistance.
Author ContributionsConceived and designed the experiments: HK WG MH. Performed the experiments: HK WG. Analyzed the data: HK WG MH. Wrote the manuscript: HK WG MH. Scientific supervision: MH. References1.
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