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, 15 October 2006, Pages 200–207
An activity-dependent assay for ricin and related RNA N-glycosidases based on electrochemiluminescence, , , , , a United States Army Medical Research Institute of Infectious Diseases, Frederick, MD, USAb BioVeris Corp., Gaithersburg, MD, USASynthetic biotinylated RNA substrates were cleaved by the combined actions of ricin holotoxin and a chemical agent, N,N&-dimethylethylenediamine. The annealing of the product with a ruthenylated oligodeoxynucleotide resulted in the capture of ruthenium chelate onto magnetic beads, enabling the electrochemiluminescence (ECL)-based detection of RNA N-glycosidase activities of toxins. ECL immunoassays and the activity assay exhibited similar limits of detection just below signals with 0.1 ng/ the ECL response was linear as the ricin concentration increased by two orders of magnitude. Activities were detected with other adenine-specific RNA N-glycosidases, including Ricinus communis agglutinin (RCA), saporin, and abrin II. The substrate that provided the greatest sensitivity was composed of a four-residue loop, GdAGA, in a hairpin structure. When the 2&-deoxyadenosine (dA) was substituted with adenosine (A), 2&-deoxyinosine, or 2&-deoxyuridine, toxin-dependent signals were abolished. Placing the GdAGA motif in a six-residue loop or replacing it with GdAdGA or GdAAA resulted in measurable activities and signal patterns that were reproducible for a given toxin. Data indicated that saporin and abrin II shared one pattern, while ricin and RCA shared a distinct pattern. A monoclonal antibody that enhanced the activities of ricin, RCA, and abrin II to different extents, thus improving the diagnostic potential of the assay, was identified .KeywordsRicin; Abrin; Saporin; Electrochemiluminescence; Activity; N-Glycosidase; Ribosome inactivating protein; Immunoassay; Bioterrorism
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Christian Porsch, Yuning Zhang, ?sa ?stlund, Peter Damberg, Cosimo Ducani, Eva Malmstr?m, and&Andreas M. Nystr?m*, "In Vitro Evaluation of Non-Protein Adsorbing Breast Cancer Theranostics Based on 19F-Polymer Containing Nanoparticles"
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Xianghui Zeng, Yuning Zhang, Jakob Regberg, Andreas M Nystr?m, “Update on polymer-based nanomedicine”, Book, Publisher iSmithers, In Press 2012. ISBN: 978-1- (Hardback) 978-1- (ebook).
Pop Sci Nanomedicine as the future therapeutics for cancer. Patient Journal for Gynecological cancers (Gynsam posten). Published 2012 (in Swedish)
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(2012) “Linear dendritic polymeric amphiphiles as carriers of doxorubicin – in vitro evaluation of biocompatibility and drug delivery”. J Pol Sci. Part A: Polymer Chemistry. 50:217-226. [Epub 2011 Oct 5]. DOI: 10.1002/pola.25008
Malkoch M, Malmstr?m E,&Nystr?m AM, 6.4 Topology, Dendrimers-Properties and application&In:&Matyjaszewski K and M?ller M (eds.) Polymer Science: A Comprehensive Reference, Vol 6, pp. 113176. Amsterdam: Elsevier BV
Bussy C, Alexiou C, Petros RA, Nystr?m AM, Kostarelos K, “Chapter XII: Therapeutic applications of engineered nanomaterials”, in Adverse Effects of Engineered Nanomaterials: a Disease-Oriented Approach., Elsevier. Eds. B Fadeel, A Pietroiusti, A Shvedova. In press 2012.
(2011) "The importance of chemistry in creating well-defined nanoscopic embedded therapeutics: Devices capable of the dual functions of imaging and therapy". Acc Chem Res, 44:969-978 [Epub 2011 Jun 15]. DOI:10.1021/ar200097k &
(2011) "Anti-biofouling hybrid dendritic Boltorn/star PEG thiol-ene crosslinked networks". ACS Applied Materials 3(6): [Epub 2011 Jun 6]. DOI: 10.1021/am200337q
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(2010) "Poly(ethylene glycol)-based thiol-ene hydrogel coatings - curing chemistry, aqueous stability, and potential marine antifouling applications". ACS App Mater Interfaces. 2:903-912 DOI 10.1021/am900875g
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(2008) &“Thiol-functionalized shell crosslinked knedel-like (SCK) nanoparticles:& A versatile entry for their conjugation with biomacromolecules”. Tetrahedron 64:.
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Center publications
2015. "Organic Bioelectronic Tools for Biomedical Applications" Electronics. 4(4): 879-908
. 2015. "Integrated patophysiology of pyelonephritis" Microbiol Spectrum 3(5): UTI-. doi:10.1128/microbiolspec.UTI-
. 2015. "Safety and Efficacy of Composite Collagen-Silver Nanoparticle Hydrogels as Tissue Engineering Scaffolds" Nanoscale. 7(44):
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&2014. "Polycaprolactone-thiophene-conjugated carbon nanotube meshes as scaffolds for cardiac progenitor cells" J Biomed Mater Res B Appl Biomater, 102(7): 1553-61. doi: 10.1002/jbm.b.33136.
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&2013. "Organic bioelectonics-Novel applications in biomedicine"&Biochim Biophys Acta. (83–4406.
&2013. "Organic bioelectronics for electronic-to-chemical translation in modulation of neuronal signaling and machine-to-brain interfacing", Biochim Biophys Acta.&(34-4344
Pontus Lundberg, Nathaniel A. Lynd, Yuning Zhang, Xianghui Zeng, Daniel V. Krogstad, Tim Paffen, Michael Malkoch,&Andreas M. Nystr?m&and Craig J. Hawker. 2013. “pH-triggered self-assembly of biocompatible histamine-functionalized triblock copolymers”,&Soft Matter (9)&82-89.
Christian Porsch, Yuning Zhang, ?sa ?stlund, Peter Damberg, Cosimo Ducani, Eva Malmstr?m, and&Andreas M. Nystr?m.&2013. “19F-based Polymer Nanoparticles as Breast Cancer Theranostics”,&Part. Part. Syst. Charact. (30) 381-390.
Xianghui Zeng, Yuning Zhang, Arpit Sand, Andreas M. Nystr?m, Research Highlight, Nanomedicine, ),
Xianghui Zeng, Yuning Zhang, and Andreas M. Nystr?m, "Endocytic uptake and intracellular trafficking of bis-MPA based hyperbranched copolymer micelles in breast cancer cells",&Biomacromolecules, 14-3822
Yong-Xing Zhao, Alan Shaw, Xianghui Zeng, Andreas M. Nystr?m&and Bj?rn H?gberg, “A DNA origami delivery system for cancer therapy with tunable release properties”,&ACS Nano,&),
Michael Malkoch, Andreas M. Nystr?m, “Biocompatible dendritic building blocks for advanced biomedical research”, Material Matters, ), 47-49
Xianghui Zeng, Yuning Zhang, Jakob Regberg, Andreas M Nystr?m, “Update on polymer-based nanomedicine”, Book, Publisher iSmithers, In Press 2012. ISBN: 978-1- (Hardback) 978-1- (ebook).
Udekwu KI & Levin BR (2012) "Staphylococcus aureus in continuous culture: a tool for the rational design of antibiotic treatment protocols".PLoS ONE PONE-D-12-.1371/journal.pone.0038866. May 13 2012.
(2012) “Fitness cost: a bacteriological explanation for the demise of the first international methicillin-resistant Staphylococcus aureus epidemic“. J Antimicrob Chemother. ):1325-32. Epub 2012 Feb 29.
(2012) “The biocompatibility and antibacterial properties of collagen-stabilized, photochemically prepared silver nanoparticles”,&Biomaterials. ):4947-56. Epub Apr 10.
(2012) “Intravital models of infection lay the foundation for tissue microbiology” Future Microbiology 7:519-33
(2012) ”Multiphoton microscopy applied for real-time intravital imaging of bacterial infections in vivo”. Meth Enzymol. 506:35-61
(2012) "Safety assessment of nanomaterials: Implications for nanomedicine". J Controlled Rel.161:403-408 [Epub 2012 25 Jan]. doi: 10.1016/j.jconrel.
(2012) "Tissue microbiology emerging". Curr Opin Microbiol 15:1-2. [Epub 2011 Dec 29]
(2012) "Tissue microbiology provides a coherent picture of infection." Curr Opin Microbiol. 15:15-22. [Epub 2011 Nov 7]. DOI 10.1016/j.mib..
(2012) "Stability and biocompatibility of a library of polyester dendrimers in comparison to polyamidoamine dendrimers". Biomaterials 33(7):1970-81. [Epub 2011 Dec 15]. doi:10.1016/j.biomaterials.
(2012) “Hyperbranched copolymers micelles as delivery vehicles of doxorubicin in breast cancer cells”. & J Pol Sci. Part A: Polymer Chemistry. 50:280-288. [Epub 2011 Oct 6]. DOI: 10.1002/pola.25027
(2012) “Linear dendritic polymeric amphiphiles as carriers of doxorubicin – in vitro evaluation of biocompatibility and drug delivery”. J Pol Sci. Part A: Polymer Chemistry. 50:217-226. [Epub 2011 Oct 5]. DOI: 10.1002/pola.25008
Richter-Dahlfors A, Berggren M. “Organic Bioelectronics” In: Encyclopedia of nanotechnology , SpringerVerlag 2012.&ISBN 978-90-481-9750-7
Malkoch M, Malmstr?m E, Nystr?m AM, 6.4 Topology, Dendrimers-Properties and application&In: Matyjaszewski K and M?ller M (eds.) Polymer Science: A Comprehensive Reference, Vol 6, pp. 113176. Amsterdam: Elsevier BV
Bussy C, Alexiou C, Petros RA, Nystr?m AM, Kostarelos K, “Chapter XII: Therapeutic applications of engineered nanomaterials”, in Adverse Effects of Engineered Nanomaterials: a Disease-Oriented Approach., Elsevier. Eds. B Fadeel, A Pietroiusti, A Shvedova. In press 2012.
Editorial in Curr Opin Microbiol
Tissue microbiology emerging - Editorial overview
Agneta Richter-Dahlfors and Guillaume DuménilCurr Opin Microbiol (-15
Over the past twenty years the field of microbial infections has made spectacular progress with the recognition of the intense and sophisticated cross-talk between bacterial pathogens and the infected host. These advances result largely from appreciating that the properties of bacteria need to be studied in the context of their interaction with host cells. Cellular microbiology studies as they were defined by Cossart et al. (‘Cellular microbiology emerging’, Science 5–6) have yielded a wealth of information including the identification and extensive characterization of secretion systems, toxins, and adhesive structures. These cell-centered strategies allow the characterization of virulence factors at the molecular level with exquisite resolution in time and space. In parallel with these studies focused on the interaction with host cells, infectious diseases have been extensively studied in various animal models. Such studies have addressed global pathophysiological issues and are particularly efficient to follow disease progression and for identifying the sites of bacterial proliferation. Recent work have demonstrated the importance of combining these two levels of analysis to provide a detailed description of the infection process inside the animal, at the molecular level, and with high resolution.
In this issue of Current Opinions in Microbiology we have brought together scientists involved in cutting edge research on the molecular basis of host pathogens interaction in vivo, to share with us their view of this rapidly evolving field of research. We have chosen to limit the scope of the reviewsto mammalian infection models to obtain a concise view of this particular research field. We feel strongly that from this series of ‘current opinions’, an integrated, rich and exciting view of the mechanisms underlying infection, opening numerous novel avenues of research, clearly emerges. As indicated by the subtitle of this special issue, we here propose tissue microbiology as a suitable term to describe this emerging research field.
An increasing number of studies point to the importance of placing the infection in the context of the whole organism or at least in the context of the infected tissue. The fact that the infection occurs inside a whole organism has several consequences on the mechanisms underlying disease progression, which we have chosen to highlight in this issue. First, tissue architecture determines the infection process, there is tremendous diversity among the different organs, and organs are more than a juxtaposition of individual cells. Brain, blood vessels, lungs, urinary tact, gut, skin, and placenta are all well-studied infection sites with significant diversity. Throughout the organism the immune system shapes the infection process in a way that is specific to the infected organ. Depending on the site of infection pathogens need to adapt to different tissue-specific environmental factors. This includes the alteration of bacterial micro-environmental factors during progression of infection, as well as mechanical challenges, such as shear stress from vascular or renal filtrate flow. Flow induced shear stress is known to influence the molecular basis for bacterial binding to biotic and abiotic surfaces. Oxygen and iron availability represent important environmental factors that affect the infection process. Finally, bacterial infections usually occur in the presence of other bacterial species. Thus, not only is communication between microbes and host cells important, but also interbacterial and poly-microbial communication.
The concept of tissue microbiology is presented in depth in "." Curr Opin
Microbiol. 15:15-22. Richter-Dahlfors A, Rhen M, Udekwu KI.
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(2011) “Uropathogenic Escherichia coli P and Type 1 fimbriae act in synergy in a living host to facilitate renal colonization leading to nephron obstruction”PLoS Pathogens 7:e1001298.Epub 2011 Feb 24]. doi:10.1371/journal.ppat.1001298
(2011) “Comparative tissue transcriptomics reveal prompt inter-organ
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(2011) "Linear dendritic polymeric amphiphiles with intrinsic
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&2013. "Anti-Peroxyl Radical Quality and Antibacterial Properties of Rooibos Infusions and Their Pure Glycosylated Polyphenolic Constituents". Molecules 18(9), .
Udekwu KI & Levin BR (2012) "Staphylococcus aureus in continuous culture: a tool for the rational design of antibiotic treatment protocols".PLoS ONE PONE-D-12-.1371/journal.pone.0038866. May 13 2012.
(2012) “Fitness cost: a bacteriological explanation for the demise of the first international methicillin-resistant Staphylococcus aureus epidemic“. J Antimicrob Chemother. ):1325-32. Epub 2012 Feb 29.
(2012) “The biocompatibility and antibacterial properties of collagen-stabilized, photochemically prepared silver nanoparticles”,&Biomaterials. ):4947-56. Epub Apr 10.
"Tissue microbiology provides a coherent picture of infection." Curr Opin Microbiol 2011 Nov 7 [Epub ahead of print]. DOI 10.1016/j.mib..
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